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Оглавление
 Contents Introduction FLUORESCENCE SPECTROSCOPY Spectra Solvatochromism Synchronous spectra Fluorescence decay and life-time Quantum yield Quenching. Stern-Volmer's equation Polarization degree and anisotropy Rotary depolarization and the Levshin-Perrin equation Resonant energy transfer Non-resonant energy transfer and exciplex emission Migration of energy and hot transfer Volumetric reabsorption Screening and reabsorption. Micro-screening and micro-reabsorption Probability of absorption. Screening hypochromizm TECHNIQUES OF EXPERIMENT Standard spectrofluorimeter Recommendations for working with spectrofluorimeter
Universal spectrofluorimeter
Phase fluorometry
Pulse fluorometry
Multi-pass cuvettes
Stopped-flow
Fluorescence correlation spectroscopy
Polarizing measurements with ConfoCor
Photochemical reactions
Photo-induced IR-emission spectroscopy
BIOLOGICAL FLUORESCENCE
Biological fluorophores
Spectral heterogeneity of tryptophan emission
Change of polarization along emission spectrum of proteins
Photo-induced conformational mobility
Deactivation of excited tryptophan residues
Resonant energy transfer in proteins
About energy transfer from tyrosine to tryptophan
Migration of energy in DNA
Spectroscopy of DNA-actinomycin complexes
Spectroscopy of the NADH dehydrogenase complex
The NADH dehydrogenase activity in mitochondrial
touch
Photo-desorption of cellular organelles
Photo-desorption of NADH from alcohol dehydrogenase
Comparison of protomitochondria and mitochondria
Changes of fluorescence of proteins and NADH at functioning of mitochondria
Loss of flavin from the NADH dehydrogenase complex of mitochondria 142
Releasing out of FAD from bacterial electron-transport flavoproteins
Chaperon complex SecB - RBPTI
Thermo- and photo-induced aggregation of a-crystalline
How a-crystallin prevents the aggregation of insulin
Excimers and dimers of pyrene in membranes
Pyrene as the indicator of oxygen and polarity
Fluorescent pharmacology
REFERENCES
Acknowledges
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